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Wednesday, April 22, 2020 | History

2 edition of Protoplast fusion in Cryptococcus laurentii found in the catalog.

Protoplast fusion in Cryptococcus laurentii

Shamin Abdul Samad

Protoplast fusion in Cryptococcus laurentii

  • 133 Want to read
  • 38 Currently reading

Published .
Written in English

    Subjects:
  • Cryptococcus laurentii,
  • Genetic engineering

  • Edition Notes

    Statementby Shamim Abdul Samad
    The Physical Object
    Paginationv, 41 leaves :
    Number of Pages41
    ID Numbers
    Open LibraryOL14056270M

    Protoplasts are obtained from two different cells to fuse and form a hybrid protoplast which is called protoplast fusion. In other words protoplast fusion which is also called as somatic fusion is a type of of genetic modification in plants by which two different species of plants are fused together and a new hybrid plant is formed with the characteristics of g: Cryptococcus laurentii. tococcus laurentii (Kufferath) Skinner, During light microscopic studies of the haemolymph of S. entomon (Linnaeus, ) that have been conducted for many years, yeast-like fungi were observed for the first time in some specimens collected in the autumn of A microscopic examination of the haemolymph of S. entomon collected in the. Start studying Microbiology Chapter 9. Learn vocabulary, terms, and more with flashcards, games, and other study tools.


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Protoplast fusion in Cryptococcus laurentii by Shamin Abdul Samad Download PDF EPUB FB2

Hence, protoplast fusion is of current interest because of its applications in pure and applied genetics. Protoplast fusion in Cryptococcus laurentii book Protoplast fusion technology is applied for developing interspecific, intraspecific and intra-generic suprahybrids with higher potentiality than their parental strains.

Protoplast fusion has proved a feasible method for inter-specific and inter-generic hybridization for strain improvement among edible. Protoplast fusion A loopful of culture was inoculated into ml flask containing 50 ml of Tryptic Soy Broth (TSB) medium and incubated on rotary shaker at rpm at 30°C.

Protoplasts were made from cells taken from mid of exponential phase. Five ml of broth was centrifuged at. Fungal Protoplast: A Biotechnological Tool - CRC Press Book A detailed collection of the results obtained during the long history of the fungal protoplast work. Fusion of protoplast takes place during slow elusion of PEG with liquid culture medium.

PowerPoint Presentation: Carrot protoplast can be fused by 28% PEG and fusion can be promoted by ca++ ion at concentration of mM. But higher concentration of Ca++ ion has been considered beneficial. In some studies, high PH/Ca++ and PEG method Missing: Cryptococcus laurentii.

Somatic Embryogenesis Somatic Hybrid Protoplast Fusion Protoplast Isolation Protoplast Culture. These keywords were added by machine and not by the authors.

This process is experimental and the keywords may be updated as the learning algorithm improves. This is a preview of subscription content, log in to check by: 4.

Protoplast fusion technique involves the fusion of protoplasts, i.e., cells devoid of cell wall, from different partners resulting in the formation of homokaryons (if fusion occurs between protoplasts from the same genotype) or heterokaryons, when fusion occurs between protoplasts of divergent genotypes.

Plants regenerated from such fusion. Protoplast Fusion and Growth of Hybrid Cells Table 6. The effect of PEG Muting solution on fusion of protoplasts derived from cultured soybean cells and Vicia ha]astana leaves Eluting solution s Percentage of heterokaryocytes to total surviving protoplasts and to soybean alone b,oFile Size: 1MB.

Protoplast fusion was performed according to a modified method of (1, 2). A sample of ml of the purified protoplast suspension (1x) of strains A and R was mixed and centrifuged at rpm for 10 minutes at 15C.

The pellet was. Protoplasts from S. rolfsii re-suspended in a fusion medium in a total volume of 1ml. Electro fusion is a modern technique of pro­toplast fusion which involves the use of mild electrical fields in protoplast suspension for inducing protoplast fusion.

This technique is very easy, simple and g: Cryptococcus laurentii. PEG-mediated DNA uptake. For this, collect the protoplasts by centrifugation at 60g for 5 min. Resuspend the protoplast pellet in approx 1 mL of X-gluc solution for protoplasts and incubate them at room temperature for h.

Take a drop of protoplast suspension (e.g., 10 $) Cited by: Protoplast fusion Purified protoplasts were resuspended in 0,1 – 0,5 ml M+C medium, mesophyll and hypocotyl protoplasts were then mixed in the ratio of and the density was adjusted to – 10 6 protoplasts per 1 ml.

4 x 50 µl (4 drops) of protoplast suspension. Optimizing a protocol for protoplast fusion of Saccharomyces cerevisiae with highly osmophilic yeasts. Yeast, 33rd International specialised symposium, Abstracts. Presented at the 33rd International specialised symposium on Yeast (ISSY): Exploring and engineering yeasts for industrial : Veerle De Clercq, Sofie De Maeseneire, Bart Devreese, Wim Soetaert.

When and why are protoplast fusion or cytoplast fusion used. 8 Production of non-inbred F1 hybrids 8 Resistance transfer 8 Further applications 9 What importance does protoplast fusion have as a breeding method at present and Protoplast fusion in Cryptococcus laurentii book the future.

9 Which plants have already been bred with the help of protoplast fusion. 10File Size: KB. Protoplast fusion, including the fusion of gametic and somatic protoplasts, is also enabling the study of the inheritance of cytoplasmic controlled traits in higher plants.

ORGANELLE GENETICS in. Protoplast fusion was used to obtain hybrids between Phaffia rhodozymaand Cryptococcus laurentii or Saccharomyces cerevisiae(petite form). Hybrids were obtained which produced the carotenoids b-carotene, phytoene and phytofluene, and were respiratory-competent and Cited by: 5.

The somatic fusion process occurs in four steps: The removal of the cell wall of one cell of each type of plant using cellulase enzyme to produce a somatic cell called a protoplast. The cells are then fused using electric shock (electrofusion) or chemical treatment to join the cells and fuse together the g: Cryptococcus laurentii.

Protoplast fusion between haploid (gametic) and diploid (somatic) cells has been achieved which clearly demonstrate that fusion is independent of cell type. Protoplasts of both types are mixed in an equal proportion attaining a density of 5 x 10 4 to 2 x 10 5 protoplasts per ml.

Protoplasts density is usually determined by g: Cryptococcus laurentii. Protoplast fusion enhances antagonistic activity in Trichoderma spp. Fahmi 1,4, A. Al-Talhi 2 and M. Hassan 3,4 1 Biotechnology Department, Faculty of Science, Taif University, KSA.

Protoplast Fusion Induced by the Application of Electric Shock Fig. Fusion of protoplasts of potato and tomato, and production of hybrid plant (pomato).

Procedure for successful somatic hybridization is as below:(i) Isolation of protoplasts from suitable plants. Descriptions of Medical fungi was released in by David Ellis, Steve Davis, Helen alexiou, Rosemary Handke and Robyn bartley.

We now provide an updated third edition which includes new and revised descriptions. We have endeavoured to reconcile current morphological descriptions with more recent phylogenetic studies, howeverFile Size: 8MB. Cryptococcus uniguttulatus – The first non-neoforman Cryptococcus species found to affect human beings.

It is encountered less frequently in humans than C. albidus and C. laurentii. It is known to cause meningitis, and responds well to amphotericin B [4]. Protoplasts were successfully isolated from one-month-old leaves of in vitro-grown plantlets using an enzyme solution comprising 1% Cellulase Onozuka R, % Macerozyme, M mannitol, 10 mM CaCl H 2 O and 10 mM 2 (N-morpholino)-ethanesulfonic acid (MES) at pH   PEG (Polyethylene glycol) Fusion This chemical has been identified as a possible/potent fusogen.

It has a high molecular weight about Usually a PEG solution of about % is used for protoplast fusion. This polymer binds to the lipid membrane of cells and thus induces fusion Fusion takes place for 45 min in incubation at r.t.p Fusion takes place slowly during Missing: Cryptococcus laurentii.

Kao et t Table 4. PEG solutions for protoplast fusion Components peg solution Pla p2 p3 PEG m m peg — — m Glucose m — m CaCl2 2 H20 mM mM mM kh2po4h2o mM mM mM pH Total molality (estimated) m m m a The PI is the same as the PEG solution F4 described previously (Kao and Michayluk,Missing: Cryptococcus laurentii.

Additional comments: Cryptococcus flavescens is physiologically similar to C. laurentii, but also to other species in the Bulleromyces clade (TableTakashima et al.

Differences from C. laurentii include the inability to assimilate d-glucosamine and creatinine, and the ability to grow without added vitamins by C. flavescens.

Protoplasts-spheroplasts of a human isolate of Cryptococcus neoformans were prepared using the gut enzyme of Helix pomatia. The induction process, as studied by transmission electron microscopy, occurred in two stages.

Early in the induction process, protoplasts-spheroplasts emerged from whole cells through a break in the cell wall-capsule by: 6.

Establishment of Method for Protoplast Fusion with PEG-mediated between Jatropha curcas L. and Ricinus communis L. Nootjaree Tudses1, Siripong Premjet,2,3 and Duangporn Premjet1,3. 1Department of Agricultural Science, Faculty of Agriculture, Natural Resources and Environment, Naresuan University, Phitsanulok, Thailand.

2Department of Biology, Faculty of Science, Naresuan University File Size: 1MB. Protoplast Fusion in Fungi The essence of protoplast fusion in fungi is the improvement of strains to be used for commercial purposes.

By this method compatibility barriers l species can be overcome] In recent years, several companies have realized to exploit this technique as a breeding tool for strain development in fungi. The intraspecies protoplast fusion of P. manshurica DUCC was conducted in searching the fusant with greater inulinase production.

Inulinase on Dahlia variabilis Willd tuber from Baturraden-Purwokerto showed inulinase activity 0, IU/mL. Inulinase is an enzyme that catalyzes the hydrolysis reaction of inulin polysaccharides into fructose and or fructooligosacarides. Induced Fusion Mechanical Fusion- Physical fusion of protoplasts under microscope by using micromanipulator and perfusion micropipette Electrofusion- Fusion induced by electrical stimulation Pearl chain of protoplasts is formed by low strength electric field (10kv m -1) Fusion of protoplasts of pearl chain is induced by the application of high Missing: Cryptococcus laurentii.

It began some 15 years ago, when Gary Harman, a Cornell professor of horticultural science, and Tom Stasz, then a research associate, took two different strains of trichoderma that occur naturally in soil and, using protoplast fusion, bioengineered a new strain. The schedules are outlined below.

For protoplast fusion see chapter on Cell Fusion (modification of R. Contts from Takebe, Otsuki and Aoki, ; see Street, ). For protoplast isolation, it is always desirable to grow plants under controlled growth conditions such as – lx light intensity–16 h Missing: Cryptococcus laurentii.

Protoplast Transformation with CRISPR RNPs. In order to optimize efficient targeted mutagenesis of grapevine MLO-7 and the DIPM-1, 2, and 4 of apple gene loci, 2 × 10 5 re-suspended protoplasts were transformed with Cas9 protein and sgRNA in a ratio of, and (Woo et al., ; Subburaj et al., ).Protoplast volume μl (2 × 10 5 cells) and RNPs for example is Cited by: Protoplast, from ancient Greek πρωτόπλαστος (prōtóplastos, "first-formed"), is a biological term coined by Hanstein in to refer to the entire cell, excluding the cell wall.

Protoplasts can be generated by stripping the cell wall from plant, bacterial, or fungal cells by mechanical, chemical or enzymatic means. Protoplasts differ from spheroplasts in that their cell wall has Missing: Cryptococcus laurentii.

Protoplast fusion One drop of dense protoplast suspension was pipetted onto the middle of the microscope slide and allowed the protoplasts to settle to the bottom for 5 min. Fusion was induced by adding a drop of 40% polyethylene glycol solution to the settled protoplasts.

Treated protoplasts Missing: Cryptococcus laurentii. Protoplast fusion technology and its biotechnology Applications. Wei, W., Kunlu Wu, Yan Qin, Zhong Xie and Xinsheng Zhu. Intergeneric protoplast fusion between Kluyveromyces and S.

cerevisiae to produce sorbitol from Jerusalem : Wijanarka Wijanarka, Sri Pujiyanto, Budi Raharjo. veloped over the years, protoplast fusion continues to be widely applied for phenotypic improvement (Gong et al., ).

Despite the fact that genome shuffling originated from protoplast fusion, it is a different method when compared to the same. Traditional protoplast fusion is the fusion between two cells with different genetic traits.

lauren 03, 04 and Cr. albi 02 could be used as þ-galactosidase source in the dairy industry. Besides, they could be used in genetic improvement of industrial yeasts strains through experiments of protoplast fusion or DNA-transformation.

Biochemical characterization studies of þ-galactosidase produced by these yeasts. Cryptococcus laurentii lung abscess. Am Rev Respir Dis. Jan; (1)– Melo JC, Srinivasan S, Scott ML, Raff MJ. Cryptococcus albidus meningitis. J Infect.

Mar; 2 (1)– Polacheck I, Hearing VJ, Kwon-Chung KJ. Biochemical studies of phenoloxidase and utilization of catecholamines in Cryptococcus neoformans.

J by: Protoplast Fusion. Protoplasts were isolated from A, B, and C cells as described previously (18), but with some modifications (Cellulase Onozuka R 1% [w/v], Macerozyme R % [w/v], potassiumdextran sulfate % [w/v], sorbitol M, man-nitol M).

Afterincubationfor40min(35 C)ofthecell-enzyme mixture, the resulting protoplasts were Cited by:. protoplast fusion: the joining together of two or more PROTOPLASTS.

Often the presence of polyethylene glycol or electric fields will enhance the frequency of fusion. In the fusion unit the DNA from the component cells may undergo RECOMBINATION. This method is useful in GENETIC ENGINEERING. It may allow the exchange of DNA between unrelated Missing: Cryptococcus laurentii.Protoplast Culture and Somatic Hybridization Lecture STUDY.

PLAY. Protoplast. vary protoplast from species/genera, fusion to create new genotypes, plant regeneration (organogenesis/somatic embryogenesis), isolated in large quantities, protoplasts must be totipotent.

Ducrey LS, Sineriz F, Spencer JFT, Spencer DM () Charac- J Basic Microbiol – terization of intergeneric hybrids obtained by protoplast fusion Kurtzman CP, Fell JW (eds) () The yeasts, a taxonomic study.

between Phaaffia rhodozyma, Cryptococcus laurentii and Elsevier, Amsterdam Kurtzman CP, Phaff HJ () Molecular taxonomy.